Chromatin Immunoprecipitation:Article Title: Regenerating aged bone marrow via a nitric oxide nanopump
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication anti-LepR-biotin antibody(BAF497, R&D Systems), anti-LepR-FITC antibody (sc-8391 FITC, Santa Cruz Biotechnology), anti-OCN antibody(PA5-78870, Thermo Fisher Scientific), anti-ASL antibody(ab97370, Abcam), anti-P21 antibody(ab188224, Abcam), anti-!H2AX antibody(ab81299, Abcam),anti-IL-6 (ab290735,Abcam), anti-PERILIPIN antibody(ab316115, Abcam), anti-CD31 antibody (ab222783, Abcam), anti-LYVE1 antibody(ab14917, Abcam), anti-PERIPHERIN antibody(ab246502, Abcam), donkey anti-goat FITC antibody(ab6881, Abcam), goat anti-rabbit FITC antibody(ab6717, Abcam),anti-Lineage Cocktail Isotype Ctrl-FITC antibody(133302, BioLegend), anti-Sca-1-APC antibody(108112, BioLegend), anti-c-Kit-PE/Cyanine7 antibody(105814, BioLegend), anti-CD48-PerCP/ Cyanine5.5 antibody(103422, BioLegend), and anti-CD150-PE antibody(162606, BioLegend) All antibodies were verified by the supplier. .. The quality test data was shown on the manufacture's websites as following: anti-LepR-biotin antibody(BAF497, R&D Systems), https://www.rndsystems.com/cn/products/mouse-leptin-r-biotinylatedantibody_baf497 anti-LepR-FITC (sc-8391 FITC, Santa Cruz Biotechnology), https://www.scbt.com/zh/p/ob-r-antibody-b-3 anti-OCN antibody(PA5-78870, Thermo Fisher Scientific), https://www.thermofisher.cn/cn/zh/antibody/product/OsteocalcinAntibody-Polyclonal/PA5-78870 anti-ASL antibody(ab97370, Abcam), https://www.abcam.cn/products/primary-antibodies/argininosuccinate-lyase-antibodyab97370.html anti-P21 antibody(ab188224, Abcam), https://www.abcam.cn/products/primary-antibodies/p21-antibody-epr18021-ab188224.html anti-!-H2AX antibody(ab81299, Abcam), https://www.abcam.cn/products/primary-antibodies/gamma-h2ax-phospho-s139-antibodyep8542y-ab81299.html anti-IL-6 antibody(ab290735,Abcam), https://www.abcam.cn/products/primary-antibodies/il-6-antibody-epr23819-103ab290735.html anti-PERILIPIN antibody(ab316115, Abcam), https://www.abcam.cn/products/primary-antibodies/perilipin-1-antibody-epr28189-12ab316115.html anti-CD31 antibody(ab222783, Abcam), https://www.abcam.cn/products/primary-antibodies/cd31-antibody-epr17260-263ab222783.html anti-LYVE1 antibody(ab14917, Abcam), https://www.abcam.cn/products/primary-antibodies/lyve1-antibody-bsa-and-azide-freeab14917.html anti-PERIPHERIN antibody(ab246502, Abcam), https://www.abcam.cn/products/primary-antibodies/peripherin-antibodyepr23445-28-ab246502.html donkey anti-goat FITC antibody(ab6881, Abcam),https://www.abcam.cn/products/secondary-antibodies/donkey-goat-igg-hl-fitcab6881.html goat anti-rabbit FITC antibody(ab6717, Abcam),https://www.abcam.cn/products/secondary-antibodies/goat-rabbit-igg-hl-fitcab6717.html anti-Lineage Cocktail Isotype Ctrl-FITC antibody(133302, BioLegend), https://www.biolegend.com/en-gb/products/fitc-anti-mouselineage-cocktail-with-isotype-ctrl-5803 anti-Sca-1-APC antibody(108112, BioLegend), https://www.biolegend.com/en-gb/products/apc-anti-mouse-ly-6a-e-sca-1antibody-225 anti-c-Kit-PE/Cyanine7 antibody(105814, BioLegend), https://www.biolegend.com/en-gb/products/pecyanine7-anti-mouse-cd117-ckit-antibody-1900 anti-CD48-PerCP/Cyanine5.5 antibody(103422, BioLegend), https://www.biolegend.com/en-gb/products/percp-cyanine5-5-antimouse-cd48-antibody-5597 anti-CD150-PE antibody(162606, BioLegend),https://www.biolegend.com/en-gb/products/pe-anti-mouse-cd150-slamantibody-22382 Primary cells were obtained from the femurs of C57/BL6 mice.
Flow Cytometry:Article Title: Regenerating aged bone marrow via a nitric oxide nanopump
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication anti-LepR-biotin antibody(BAF497, R&D Systems), anti-LepR-FITC antibody (sc-8391 FITC, Santa Cruz Biotechnology), anti-OCN antibody(PA5-78870, Thermo Fisher Scientific), anti-ASL antibody(ab97370, Abcam), anti-P21 antibody(ab188224, Abcam), anti-!H2AX antibody(ab81299, Abcam),anti-IL-6 (ab290735,Abcam), anti-PERILIPIN antibody(ab316115, Abcam), anti-CD31 antibody (ab222783, Abcam), anti-LYVE1 antibody(ab14917, Abcam), anti-PERIPHERIN antibody(ab246502, Abcam), donkey anti-goat FITC antibody(ab6881, Abcam), goat anti-rabbit FITC antibody(ab6717, Abcam),anti-Lineage Cocktail Isotype Ctrl-FITC antibody(133302, BioLegend), anti-Sca-1-APC antibody(108112, BioLegend), anti-c-Kit-PE/Cyanine7 antibody(105814, BioLegend), anti-CD48-PerCP/ Cyanine5.5 antibody(103422, BioLegend), and anti-CD150-PE antibody(162606, BioLegend) All antibodies were verified by the supplier. .. The quality test data was shown on the manufacture's websites as following: anti-LepR-biotin antibody(BAF497, R&D Systems), https://www.rndsystems.com/cn/products/mouse-leptin-r-biotinylatedantibody_baf497 anti-LepR-FITC (sc-8391 FITC, Santa Cruz Biotechnology), https://www.scbt.com/zh/p/ob-r-antibody-b-3 anti-OCN antibody(PA5-78870, Thermo Fisher Scientific), https://www.thermofisher.cn/cn/zh/antibody/product/OsteocalcinAntibody-Polyclonal/PA5-78870 anti-ASL antibody(ab97370, Abcam), https://www.abcam.cn/products/primary-antibodies/argininosuccinate-lyase-antibodyab97370.html anti-P21 antibody(ab188224, Abcam), https://www.abcam.cn/products/primary-antibodies/p21-antibody-epr18021-ab188224.html anti-!-H2AX antibody(ab81299, Abcam), https://www.abcam.cn/products/primary-antibodies/gamma-h2ax-phospho-s139-antibodyep8542y-ab81299.html anti-IL-6 antibody(ab290735,Abcam), https://www.abcam.cn/products/primary-antibodies/il-6-antibody-epr23819-103ab290735.html anti-PERILIPIN antibody(ab316115, Abcam), https://www.abcam.cn/products/primary-antibodies/perilipin-1-antibody-epr28189-12ab316115.html anti-CD31 antibody(ab222783, Abcam), https://www.abcam.cn/products/primary-antibodies/cd31-antibody-epr17260-263ab222783.html anti-LYVE1 antibody(ab14917, Abcam), https://www.abcam.cn/products/primary-antibodies/lyve1-antibody-bsa-and-azide-freeab14917.html anti-PERIPHERIN antibody(ab246502, Abcam), https://www.abcam.cn/products/primary-antibodies/peripherin-antibodyepr23445-28-ab246502.html donkey anti-goat FITC antibody(ab6881, Abcam),https://www.abcam.cn/products/secondary-antibodies/donkey-goat-igg-hl-fitcab6881.html goat anti-rabbit FITC antibody(ab6717, Abcam),https://www.abcam.cn/products/secondary-antibodies/goat-rabbit-igg-hl-fitcab6717.html anti-Lineage Cocktail Isotype Ctrl-FITC antibody(133302, BioLegend), https://www.biolegend.com/en-gb/products/fitc-anti-mouselineage-cocktail-with-isotype-ctrl-5803 anti-Sca-1-APC antibody(108112, BioLegend), https://www.biolegend.com/en-gb/products/apc-anti-mouse-ly-6a-e-sca-1antibody-225 anti-c-Kit-PE/Cyanine7 antibody(105814, BioLegend), https://www.biolegend.com/en-gb/products/pecyanine7-anti-mouse-cd117-ckit-antibody-1900 anti-CD48-PerCP/Cyanine5.5 antibody(103422, BioLegend), https://www.biolegend.com/en-gb/products/percp-cyanine5-5-antimouse-cd48-antibody-5597 anti-CD150-PE antibody(162606, BioLegend),https://www.biolegend.com/en-gb/products/pe-anti-mouse-cd150-slamantibody-22382 Primary cells were obtained from the femurs of C57/BL6 mice.
Magnetic Resonance Imaging:Article Title: Regenerating aged bone marrow via a nitric oxide nanopump
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication anti-LepR-biotin antibody(BAF497, R&D Systems), anti-LepR-FITC antibody (sc-8391 FITC, Santa Cruz Biotechnology), anti-OCN antibody(PA5-78870, Thermo Fisher Scientific), anti-ASL antibody(ab97370, Abcam), anti-P21 antibody(ab188224, Abcam), anti-!H2AX antibody(ab81299, Abcam),anti-IL-6 (ab290735,Abcam), anti-PERILIPIN antibody(ab316115, Abcam), anti-CD31 antibody (ab222783, Abcam), anti-LYVE1 antibody(ab14917, Abcam), anti-PERIPHERIN antibody(ab246502, Abcam), donkey anti-goat FITC antibody(ab6881, Abcam), goat anti-rabbit FITC antibody(ab6717, Abcam),anti-Lineage Cocktail Isotype Ctrl-FITC antibody(133302, BioLegend), anti-Sca-1-APC antibody(108112, BioLegend), anti-c-Kit-PE/Cyanine7 antibody(105814, BioLegend), anti-CD48-PerCP/ Cyanine5.5 antibody(103422, BioLegend), and anti-CD150-PE antibody(162606, BioLegend) All antibodies were verified by the supplier. .. The quality test data was shown on the manufacture's websites as following: anti-LepR-biotin antibody(BAF497, R&D Systems), https://www.rndsystems.com/cn/products/mouse-leptin-r-biotinylatedantibody_baf497 anti-LepR-FITC (sc-8391 FITC, Santa Cruz Biotechnology), https://www.scbt.com/zh/p/ob-r-antibody-b-3 anti-OCN antibody(PA5-78870, Thermo Fisher Scientific), https://www.thermofisher.cn/cn/zh/antibody/product/OsteocalcinAntibody-Polyclonal/PA5-78870 anti-ASL antibody(ab97370, Abcam), https://www.abcam.cn/products/primary-antibodies/argininosuccinate-lyase-antibodyab97370.html anti-P21 antibody(ab188224, Abcam), https://www.abcam.cn/products/primary-antibodies/p21-antibody-epr18021-ab188224.html anti-!-H2AX antibody(ab81299, Abcam), https://www.abcam.cn/products/primary-antibodies/gamma-h2ax-phospho-s139-antibodyep8542y-ab81299.html anti-IL-6 antibody(ab290735,Abcam), https://www.abcam.cn/products/primary-antibodies/il-6-antibody-epr23819-103ab290735.html anti-PERILIPIN antibody(ab316115, Abcam), https://www.abcam.cn/products/primary-antibodies/perilipin-1-antibody-epr28189-12ab316115.html anti-CD31 antibody(ab222783, Abcam), https://www.abcam.cn/products/primary-antibodies/cd31-antibody-epr17260-263ab222783.html anti-LYVE1 antibody(ab14917, Abcam), https://www.abcam.cn/products/primary-antibodies/lyve1-antibody-bsa-and-azide-freeab14917.html anti-PERIPHERIN antibody(ab246502, Abcam), https://www.abcam.cn/products/primary-antibodies/peripherin-antibodyepr23445-28-ab246502.html donkey anti-goat FITC antibody(ab6881, Abcam),https://www.abcam.cn/products/secondary-antibodies/donkey-goat-igg-hl-fitcab6881.html goat anti-rabbit FITC antibody(ab6717, Abcam),https://www.abcam.cn/products/secondary-antibodies/goat-rabbit-igg-hl-fitcab6717.html anti-Lineage Cocktail Isotype Ctrl-FITC antibody(133302, BioLegend), https://www.biolegend.com/en-gb/products/fitc-anti-mouselineage-cocktail-with-isotype-ctrl-5803 anti-Sca-1-APC antibody(108112, BioLegend), https://www.biolegend.com/en-gb/products/apc-anti-mouse-ly-6a-e-sca-1antibody-225 anti-c-Kit-PE/Cyanine7 antibody(105814, BioLegend), https://www.biolegend.com/en-gb/products/pecyanine7-anti-mouse-cd117-ckit-antibody-1900 anti-CD48-PerCP/Cyanine5.5 antibody(103422, BioLegend), https://www.biolegend.com/en-gb/products/percp-cyanine5-5-antimouse-cd48-antibody-5597 anti-CD150-PE antibody(162606, BioLegend),https://www.biolegend.com/en-gb/products/pe-anti-mouse-cd150-slamantibody-22382 Primary cells were obtained from the femurs of C57/BL6 mice.
Biomarker Discovery:Article Title: Regenerating aged bone marrow via a nitric oxide nanopump
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication anti-LepR-biotin antibody(BAF497, R&D Systems), anti-LepR-FITC antibody (sc-8391 FITC, Santa Cruz Biotechnology), anti-OCN antibody(PA5-78870, Thermo Fisher Scientific), anti-ASL antibody(ab97370, Abcam), anti-P21 antibody(ab188224, Abcam), anti-!H2AX antibody(ab81299, Abcam),anti-IL-6 (ab290735,Abcam), anti-PERILIPIN antibody(ab316115, Abcam), anti-CD31 antibody (ab222783, Abcam), anti-LYVE1 antibody(ab14917, Abcam), anti-PERIPHERIN antibody(ab246502, Abcam), donkey anti-goat FITC antibody(ab6881, Abcam), goat anti-rabbit FITC antibody(ab6717, Abcam),anti-Lineage Cocktail Isotype Ctrl-FITC antibody(133302, BioLegend), anti-Sca-1-APC antibody(108112, BioLegend), anti-c-Kit-PE/Cyanine7 antibody(105814, BioLegend), anti-CD48-PerCP/ Cyanine5.5 antibody(103422, BioLegend), and anti-CD150-PE antibody(162606, BioLegend) All antibodies were verified by the supplier. .. The quality test data was shown on the manufacture's websites as following: anti-LepR-biotin antibody(BAF497, R&D Systems), https://www.rndsystems.com/cn/products/mouse-leptin-r-biotinylatedantibody_baf497 anti-LepR-FITC (sc-8391 FITC, Santa Cruz Biotechnology), https://www.scbt.com/zh/p/ob-r-antibody-b-3 anti-OCN antibody(PA5-78870, Thermo Fisher Scientific), https://www.thermofisher.cn/cn/zh/antibody/product/OsteocalcinAntibody-Polyclonal/PA5-78870 anti-ASL antibody(ab97370, Abcam), https://www.abcam.cn/products/primary-antibodies/argininosuccinate-lyase-antibodyab97370.html anti-P21 antibody(ab188224, Abcam), https://www.abcam.cn/products/primary-antibodies/p21-antibody-epr18021-ab188224.html anti-!-H2AX antibody(ab81299, Abcam), https://www.abcam.cn/products/primary-antibodies/gamma-h2ax-phospho-s139-antibodyep8542y-ab81299.html anti-IL-6 antibody(ab290735,Abcam), https://www.abcam.cn/products/primary-antibodies/il-6-antibody-epr23819-103ab290735.html anti-PERILIPIN antibody(ab316115, Abcam), https://www.abcam.cn/products/primary-antibodies/perilipin-1-antibody-epr28189-12ab316115.html anti-CD31 antibody(ab222783, Abcam), https://www.abcam.cn/products/primary-antibodies/cd31-antibody-epr17260-263ab222783.html anti-LYVE1 antibody(ab14917, Abcam), https://www.abcam.cn/products/primary-antibodies/lyve1-antibody-bsa-and-azide-freeab14917.html anti-PERIPHERIN antibody(ab246502, Abcam), https://www.abcam.cn/products/primary-antibodies/peripherin-antibodyepr23445-28-ab246502.html donkey anti-goat FITC antibody(ab6881, Abcam),https://www.abcam.cn/products/secondary-antibodies/donkey-goat-igg-hl-fitcab6881.html goat anti-rabbit FITC antibody(ab6717, Abcam),https://www.abcam.cn/products/secondary-antibodies/goat-rabbit-igg-hl-fitcab6717.html anti-Lineage Cocktail Isotype Ctrl-FITC antibody(133302, BioLegend), https://www.biolegend.com/en-gb/products/fitc-anti-mouselineage-cocktail-with-isotype-ctrl-5803 anti-Sca-1-APC antibody(108112, BioLegend), https://www.biolegend.com/en-gb/products/apc-anti-mouse-ly-6a-e-sca-1antibody-225 anti-c-Kit-PE/Cyanine7 antibody(105814, BioLegend), https://www.biolegend.com/en-gb/products/pecyanine7-anti-mouse-cd117-ckit-antibody-1900 anti-CD48-PerCP/Cyanine5.5 antibody(103422, BioLegend), https://www.biolegend.com/en-gb/products/percp-cyanine5-5-antimouse-cd48-antibody-5597 anti-CD150-PE antibody(162606, BioLegend),https://www.biolegend.com/en-gb/products/pe-anti-mouse-cd150-slamantibody-22382 Primary cells were obtained from the femurs of C57/BL6 mice.
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